Electron crystallography
Electron crystallography (also called two-dimensional microscopy) works with two-dimensional crystals of molecules that require less material and form more readily than single three dimensional crystals - required for X-ray crystallography. Using electrons instead of X-rays Electron crystallography can be applied to smaller samples than X-ray diffraction due to the strong interaction of electrons with matter. Electron crystallography can reveal shape and symmetry of assemblies mostly at an intermediate resolution and can thus provide a sort of a scaffold to be filled with X-ray structures of the single subunits.
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